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Structured Review

Merck KGaA bugbuster solution
Bugbuster Solution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bugbuster+solution/bugbuster/pm40157313-67-10-12
Average 90 stars, based on 1 article reviews
bugbuster solution - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Concentration Assay:

Article Title: Machine-Learning-Guided Mutagenesis for Directed Evolution of Fluorescent Proteins.
Article Snippet: .. After incubating for 3 h at 37 °C, isopropyl-1-thio-L-D- Page 13 of 32 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 galactopyranoside was added to each well to a final concentration of 1 mM to induce the GFP variant expression, and cells were incubated for a further 6 h. The harvested cells were collected and then 200 μL of BugBuster solution (MerK, NJ, USA) was added. ..

Variant Assay:

Article Title: Machine-Learning-Guided Mutagenesis for Directed Evolution of Fluorescent Proteins.
Article Snippet: .. After incubating for 3 h at 37 °C, isopropyl-1-thio-L-D- Page 13 of 32 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 galactopyranoside was added to each well to a final concentration of 1 mM to induce the GFP variant expression, and cells were incubated for a further 6 h. The harvested cells were collected and then 200 μL of BugBuster solution (MerK, NJ, USA) was added. ..

Expressing:

Article Title: Machine-Learning-Guided Mutagenesis for Directed Evolution of Fluorescent Proteins.
Article Snippet: .. After incubating for 3 h at 37 °C, isopropyl-1-thio-L-D- Page 13 of 32 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 galactopyranoside was added to each well to a final concentration of 1 mM to induce the GFP variant expression, and cells were incubated for a further 6 h. The harvested cells were collected and then 200 μL of BugBuster solution (MerK, NJ, USA) was added. ..

Incubation:

Article Title: Machine-Learning-Guided Mutagenesis for Directed Evolution of Fluorescent Proteins.
Article Snippet: .. After incubating for 3 h at 37 °C, isopropyl-1-thio-L-D- Page 13 of 32 ACS Paragon Plus Environment ACS Synthetic Biology 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 galactopyranoside was added to each well to a final concentration of 1 mM to induce the GFP variant expression, and cells were incubated for a further 6 h. The harvested cells were collected and then 200 μL of BugBuster solution (MerK, NJ, USA) was added. ..

Recombinant:

Article Title: Characterization of a lipocalin-like molecule from Dermanyssus gallinae as a potential vaccine antigen.
Article Snippet: Poultry red mites (PRMs, Dermanyssus gallinae) are hematophagous ectoparasites of chickens that pose a significant threat to the egg-laying industry.. The emergence of acaricide-resistant PRMs raises the demand for alternative control approaches such as vaccination.. However, several vaccine antigens have failed to suppress the growth of PRM populations in field trials due to difficulties in maintaining antibody levels.

other:

Article Title: Functional Characterization of the Unique Terminal Thioesterase Domain from Polymyxin Synthetase.
Article Snippet: Polymyxins remain one of the few antibiotics available for treating antibiotic resistant bacteria.. Here we describe polymyxin B thioesterase which performs the final step in polymyxin B biosynthesis.. Isolated thioesterase catalyzed cyclization of an Nacetylcystamine polymyxin B analogue to form polymyxin B.

Protease Inhibitor:

Article Title: Engineered Fully Human Single-Chain Monoclonal Antibodies to PIM2 Kinase
Article Snippet: .. Bacterial cells harvested from the cultures were lysed by using 0.5 mL BugBuster ® solution (Merck KGaA) supplemented with 25 U/mL Benzonase ® (Merck KGaA) and 1:200 protease inhibitor cocktail set III (Merck KGaA). ..

Cell Culture:

Article Title: Creation of haemoglobin A1c direct oxidase from fructosyl peptide oxidase by combined structure-based site specific mutagenesis and random mutagenesis
Article Snippet: .. Cultured cells were collected by centrifugation and then lysed using 50 L of BugBuster solution (Merck Millipore, MA, USA). ..

Centrifugation:

Article Title: Creation of haemoglobin A1c direct oxidase from fructosyl peptide oxidase by combined structure-based site specific mutagenesis and random mutagenesis
Article Snippet: .. Cultured cells were collected by centrifugation and then lysed using 50 L of BugBuster solution (Merck Millipore, MA, USA). ..



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